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Open the card, open the menu in the upper right corner and pick Delete card. Confirm with Yes, delete card. Studio returns to the Cards page. Delete card dialog
Deleting is permanent. To take a card out of use but keep it, archive it instead.

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When deletion is blocked

The menu item is disabled when the card is used by a destination, a segment or a relation. To delete such a card, first remove it from every destination and segment, and delete the relations it takes part in. The usage widgets on the card page list everything you need to clear.
Deletion is also blocked while the card is Updating.
A card’s status does not by itself prevent deletion. An Active card with no segments, destinations or relations can be deleted; an unused Archived card can be deleted too.